Escherichia coli strain BL21 is one of the widely used bacterial hosts for high-level recombinant protein production and for other applications. Here, we present the complete genome sequence of a commercial version of the Escherichia coli BL21 strain.
BL21 (DE3) Expression from T7 promoter, tight regulation: BL21 (DE3) pLysS: Expression from T7 promoter with codon bias correction: BL21 codon plus, Rosetta: Improved disulphide bond formation: Origami: Fast cloning (due to quick cell growth) Mach1
In this work, four bacterial strains, BL21 (DE3), BL21 (DE3) pLysS, Rosetta, and Arctic Express, were chosen for parallel expression trials along with BL21 (DE3) complemented with folding chaperones DnaJ/K and GroEL/ES to compare their performance in producing soluble and active human kinases.
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difference between rosetta and bl21
